Acquiring a low volume dispenser is an investment that should be leveraged. While in the past, you may have had to select a spotter based on a particular technology or dispense volume range, the latest generation of systems now offer interchangeable nozzles.
For example, you may have a current need to dispense 20 nl volumes for a protein array. However, you may also have on your radar to explore DNA arrays using much smaller spots and requiring 30 picoliter droplets. Or you may be looking at dispensing protein solutions that are too viscous for piezo dispensers.
Now, with the same system, you can explore all of those technologies, thus maximizing results you can obtain from the single instrument acquisition.
Four spotting technologies are available on M2-Automation systems for optimal dispensing: piezo-based, solenoid-based, pin-based, and a unique cartridge with bulk dispensing capabilities. These can be combined on the same system, for unparalleled flexibility and ROI
M2 Automation Systems for Spotting and Low Volume Depositions
Arraying, Spotting, Patterning, Depositing Materials onto almost any surfaces
Over 15 years of continuous improvements have resulted in the best hardware and software systems for ultra-low volume high-accuracy and precision liquid handling. From their origin at the Max Planck Institute for Molecular Genetics in Berlin, the engineering founders have sought the very best technological solutions to achieve robust operations. The main application is the production of microarrays, made up of DNA, aptamers, proteins, glycans, or other materials.
For example, instead of trying to solve all deposition tasks using a single dispensing technology, they produced systems that can easily interchange several dispense heads. These included piezo-based, solenoid-based, pin-based, and their own proprietary cartridges with mutilple operation modes. You can get an overview of some of these features here.
Dr Eckard Nordoff, one of the founders, is one the inventor of the original piezo dispensing nozzle used on arrayer. He has continued to further optimize this technology and the resulting Piezo Dispenser M2 PDMDs can dispense droplets as small as 20 picoliter in a robust manner.
The instrumentTWO-300P series is M2-Automation’s newest addition to the family. Providing its users with a broad range of custom-tailored solutions, the iTWO™-300P is ready for any challenge to come. With a larger area for production (220 x 300 mm) and an improved repeated positioning (<5 µm) it is the ideal choice for research and development and small-scale production. Of course, additional new options like the heatable M2-Dispenser for higher viscous samples, the contact-based Pin-Dispenser, a vacuum table, humidity control of +/- 1% are only a few options that you can combine with our established technologies like the Quattro-Dispensing or the online-QC and a variety of camera options.
The instrumentONE series is an ultra-fast, high-precision, non-contact liquid handling systems that meet and grow with your needs. It enables flexibility in pattern design and high-speed printing with micro-dispensers through the use of a unique Triple-Jet technology, online degassing and online quality control.
The Quattro-Dispensing Technology of the iONE makes array production easier as a result of the simplified liquid path handling.
As a testament to the flexibility of its design, it is available in four different sizes, a variety of target capacities and with a selection of essential components, which makes it ideal for many applications in pL, nL, μL and low mL volume ranges.
- Target: 60–156 glass slides, 8-24 MTPs and 3 wafers (12’’)
Dual Head Technology, which is perfect for multitasking functions, enables massive sample spotting, recovery of single sample spotting and optical inline quality control. The technology combines two different micro-dispensers in one, single instrument:
- Pin Driven Micro-Dispenser(PinDMD) for pico- to low nanolitre applications, with Dual Head and inline QC for an optimal microarray result
- Piezo Driven Micro-Dispenser (PDMD) for pico- to low nanolitre applications
M2-Automation’s PinDMD technology is made for low volume protein and DNA microarray printing on different coolable targets under controlled conditions (Climate Control system for controlling temperature, humidity and DEW point).
The instrumentSIX is a flexible system with Ultrasonic, Wash and Dry station that are perfect for the cleaning of the micro-dispensers. The instrument can be equipped with a flexible amount of target glass- or plastic-slides, MTP holders and two coolable sources. The head camera performs inline quality control for finding missed spots, which are respotted with the second single pin head in order to achieve the highest microarray quality.
The instrumentZERO series contains everything you value about M2-Automation’s technology and combines it with a fully flexible, scalable and highly automated production environment. Without the need to change or reprogram the entire setting during production, the remote-controlled software handles a variety of different products, and protocols at ease.
From a few dozen to several thousand units produced per day, the almost maintenance free iZERO™ reduces the changeover time to a minimum while maximizing its productive time - Making it the obvious choice to spot your future.
"The quality, flexibility, wide volume range and ease of software use, was our main priority to choose M2-Automation Dispensing Technology. The outstanding application and after sales support shows the attitude of the M2 team commitment. After experiencing other dispensing technologies, M2 technology is the right choice for our research work." Dr Peter Miethe, CEO of fzmb GmbH, Bad-Langensalza, Germany.
"M2-Automation dispensing technology offers high-precision, speed and good reproducibility for glycan and glycopeptide microarray spotting. This is critical for studies of glycan-protein interactions or for mapping of antibodies recognizing glycan or glycopeptide specific binding epitopes. Besides that, M2 offers an outstanding service and support team." - Associtate Professor Ulrika Westerlind, Dept of Chemistry, Umea University, Sweden.
"As a manufacturer of blood analysis technology, we rely on precise and robust manufacturing technologies. With M2, we have found a reliable partner who has supported us very well in the qualification of the dispensing system. Any special requests were easily possible, so that we could implement our ideal dispensing request without compromise. We also greatly appreciate the quick service from M2 for subsequent conversions." - Stefan Frenzel, EKF-diagnostics GmbH, Germany.l